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Cascada™ BIO- and AN-Water Systems: UV Bacterial Challenge
  Cascada™ BIO- and AN-Water Systems: UV Bacterial Challenge


Introduction
The Cascada BIO- and AN-water Systems incorporate a stainless steel chamber with a UV lamp emitting both 185 nm and 254 nm to give enhanced photo-oxidation of residual organic impurities and bactericidal effectiveness.

When challenged with 1 x 107 CFU/mL of typical waterborne bacteria under standard flow conditions, the UV in the Cascada Lab Water Systems produced water with a bacteria content at or below the detection limit of 0.01 CFU/mL — a log10 reduction of 9.

These results demonstrate that even in the unlikely event of a very high level of bacteria reaching the UV, there would be no breakthrough into the product water.


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Results
Sample Bacteria Concentration (CFU/mL)
Pre UV 1.0 x 107
Post UV 0 minutes < 0.01
Post UV 1 minute < 0.01
Post UV 2 minutes 0.01
Post UV 5 minutes 0.01
Log10 Reduction > 9

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Method
A challenge was prepared by inoculating 200 mL of sterile peptone water with 10 mL of purified water, agitating and incubating for 2 days at 27 °C to produce a culture of bacteria commonly found in purified water. After dilution to 25 liters, the water contained 1 x 107 CFU/mL.

This challenge was pumped through the UV at flow rates of up to 2 L/min and samples taken before and after the UV. The bacteria present were measured by the membrane filtration technique and incubation on R2A agar at 27 °C for 5 days.


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