Jumbosep™ Centrifugal Devices

Convenient and reliable concentration, purification, and diafiltration of 15 to 60 mL biological samples

Concentrate 60 mL sample volumes to 4 mL in 30 minutes.
High recoveries, typically greater than 90%.
Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding.
Versatile. Available in a variety of MWCOs color coded for easy identification.
Built-in deadstop prevents spinning to dryness.
Unique sealing mechanism prevents retentate leakage and filtrate contamination.
Economical. Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused.

Replaces dialysis, chemical precipitation, and lyophilization in the following applications:

Concentrate and desalt proteins and nucleic acids.
Buffer exchange or salt removal of chromatography eluates and gradient fractions.
Separate biomolecules from cell culture supernatants.
Concentrate or remove viruses.
Crude fractionation of dilute protein mixtures.
Remove debris and particulates from cell lysates.

Materials of Construction
Filter Media: Omega™ membrane (low protein-binding, modified polyethersulfone)
Sample Reservoir and Filtrate Receiver: Polysulfone
Sample Reservoir Cap: Polyethylene
Insert Without Membrane: High density polyethylene
Filtrate Receiver Cap and Insert Release: Polypropylene

Effective Filtration Area
15.2 cm²

Dimensions
Outside Diameter (maximum): 6 cm (2.4 in.)
Overall Height (fully assembled with cap): 11.3 cm (4.5 in.)

Capacities
Maximum Sample Volume: 60 mL
Final Concentrate Volume: 3.5 - 4 mL
Maximum Filtrate Receiver Volume: 60 mL
Hold-up Volume (membrane/support): 0.2 mL

Operating Temperature Range
0 - 40 °C (32 - 104 °F)

pH Range
1 - 14

Centrifuge
Swinging bucket rotor is required that accepts flat-bottomed 250 mL bottles and is capable of spinning at up to 3,000 x g

Sanitization
Provided non-sterile; may be sanitized by filtering 70% ethanol through the device prior to use.

Jumbosep Device Reduces Processing
Time Over Other Devices

1 mg/mL BSA solution was processed in each of the above
devices until a 15-fold concentration was achieved.

Concentration Time

Concentrate dilute protein samples in less than 30 minutes
with 10, 30, and 100K Jumbosep devices.

Centrifuge

Swinging bucket rotor is required that accepts 250 mL bottles and is capable of spinning at up to 3000 x g

Sanitization

Provided non-sterile; may be sanitized by filtering 70% ethanol through the device prior to use

Testing info by rg

Jumbosep Centrifugal Device Starter Kits
Part Number	Description	Pkg	Price	Qty
FD000K65	Generic starter kit, (no membrane inserts)	4/pkg	135.63
FD003K65	3K starter kit, gray	4/pkg	180.89
FD010K65	10K starter kit, blue	4/pkg	180.89
FD030K65	30K starter kit, red	4/pkg	180.89
FD100K65	100K starter kit, clear	4/pkg	180.89
FD300K65	300K starter kit, orange	4/pkg	180.89
Jumbosep Centrifugal Device Membrane Inserts
OD003C65	3K membrane insert, gray	12/pkg	186.30
OD010C65	10K membrane insert, blue	12/pkg	186.30
OD030C65	30K membrane insert, red	12/pkg	186.30
OD100C65	100K membrane inser, clear	12/pkg	187.67
OD300C65	300K membrane insert, orange	12/pkg	187.67
Accessory Products
FD001X65	Filtrate receiver and cap	12/pkg	119.76
FD002X65	Sample reservoir and cap	12/pkg	161.73
FD003X65	Insert release	24/pkg	107.78

Contact Customer Service at 800-521-1520 for possible lead-time and date item will be available to ship.