Acrodisc Syringe Filters with GHP MembraneMaterials of ConstructionFilter Media: GH Polypro (GHP) membrane (hydrophilic polypropylene)
Housing: Polypropylene
GxF Prefilter: Binder-free borosilicate glass
Pore Size0.2 and 0.45 µm
Effective Filtration Area13 mm: 1.0 cm
225 mm PSF: 3.9 cm
2
Sample Volume13 mm: < 10 mL
25 mm PSF: < 150 mL
Inlet/Outlet Connections
13 mm: Female Luer-Lok* inlet, minispike outlet
25 mm PSF: Female Luer-Lok* inlet, male slip luer outlet
Typical Hold-up Volume
(with air purge)
13 mm: < 14 µL
25 mm PSF: < 125 µL
25 mm PSF GxF: < 200 µL
Maximum Operating Temperature
55 °C (131 °F)
Maximum Operating Pressure
13 mm: 6.3 bar (630 kPa, 90 psi)
25 mm PSF: 4.1 bar (410 kPa, 60 psi) at
21 - 24 ºC (70 - 75 ºF); 2.1 bar (210 kPa, 30 psi) at 55 ºC (131 ºF)
Typical Water Flow Rate
mL/min at 0.7 bar (70 kPa, 10 psi)
13 mm, 0.2 µm: 17
13 mm, 0.45 µm: 28
mL/min at 2.1 bar (210 kPa, 30 psi)
25 mm PSF, 0.2 µm and
GxF/0.2 µm: 175
25 mm PSF, 0.45 µm and
GxF/0.45 µm: 195
GH Polypro (GHP) Membrane Disc Filters
Filter Media
Hydrophilic polypropylene
Pore Size
0.2 and 0.45 µm
Typical Thickness
0.2 µm: 101 µm (4.0 mils)
0.45 µm: 114 µm (4.5 mils)
Typical Water Flow Rate
mL/min/cm2 at 0.7 bar (70 kPa, 10 psi)
0.2 µm: 20
0.45 µm: 31
Maximum Operating Temperature
55 °C (131 °F)
Minimum Bubble Point — Water
0.2 µm: 2.9 bar (290 kPa, 42 psi)
0.45 µm: 1.38 bar (138 kPa, 20 psi)
GH Polypro (GHP) Membrane is Extremely Low in Biomolecule Binding

125I-labeled BSA (1.6 µg) or 32P-labeled DNA (500 ng) were diluted to 5 mL in PBS (BSA) or Tris-EDTA (DNA) and filtered through a 13 mm disc of the indicated membrane. Filtration was carried out using a 10 mL syringe at a flow rate of 1.0 mL/min. Binding was determined by comparing the amount of radioactivity remaining in the membrane (triplicate) to the activity of the starting material by placing the disc or solution directly into scintillation cocktail and counting in a scintillation counter. Biodyne B membrane is designed for biomolecule binding and was used as a positive control.