AcroPrep™ 96 Filter Plates, 350 µL, for Sample Preparation and Detection

Excellent for use in both molecular biology and analytical applications

Plates are constructed from chemically-resistant, biologically-inert polypropylene with a clear polystyrene lid.
Robotic-friendly design has single-piece construction, designed in accordance with the ANSI/SBS X-2004 standards. Plates are stackable with and without lids.
Eliminates crosstalk between wells. Membranes are individually sealed in each well with a proprietary sealing process and each well has a fluid deflector.
A serialized barcode label allows for the use of automated tracking systems and identifies the membrane type.
Available in a variety of membrane configurations to meet the requirements of numerous applications.
Available in a variety of housing colors to help optimize detection.

Products in this data sheet may be covered by one or more patents including EP 1,056,540; US 6,878,343; US 7,135,117; US 7,371,325; EP 0,640,007; US 5,209,849.

Applications by Membrane Type

GHP Membrane

Cleavage reactions
Receptor: ligand screens
Bead-/resin-based purification
Plasmid lysate clearing
Isolate DNA from agarose gel slices
HPLC sample prep and clarification
Metabolic studies
Applications that use harsh organics
Time resolved fluorescence (TRF)
Microfiltration
Molecular synthesis
Enzyme assays

Bio-Inert® Membrane

Purification of oligos from polyacrylamide gels
General sample prep
Metabolic assays
Bead-based assays

PTFE Membrane

Solubility studies
Cleavage reactions

Glass Fiber Media

Gross fractionation
Lysate clarification
Isolation of RNA, gDNA and plasmid from culture
Radiolabeled cell assays
Enzyme

Glass Fiber Over Bio-Inert Membrane

Clarification of cell lysates and tissue homogenates
Preparation of proteinaceous solutions
Applications that require prefiltration

Omega™ Ultrafiltration Membrane

Size exclusion
Concentrate, purify, and desalt proteins, peptides, oligos, DNA, and RNA
Recover proteins, oligonucleotides and RNA from polyacrylamide gels
Nucleic acid purification
Compound purification
Drug plasma binding
Protein binding studies

Supor® Membrane

Lysate clearance
Bead-/resin-based applications
General filtration

Polypropylene over Supor Membrane

Fast lysate clearance at low salt conditions
Clarification of high particulate-laden solutions
Preparation of proteinaceous solutions

Mustang™ Ion Exchange Membrane

Plasmid purification
Ion exchange applications
Oligonucleotide purification
Protein fractionation
Protein capture
Antibody purification

54 µm Polyester Screen

Sample preparation for cell analysis/flow cytometry assays
Separation of cell, organisms and large particulates

Materials of Construction
Filter Media: Bio-Inert® (modified low protein-binding Nylon), GHP (hydrophilic polypropylene), Omega™ (modified polyethersulfone), PTFE (hydrophobic PTFE), Supor® (hydrophilic polyethersulfone), and Mustang™ Q and S membranes; and glass fiber (borosilicate glass without binder)
Plate Housing: Polypropylene
Lid: Polystyrene

Dimensions
Height: 1.7 cm (0.67 in.) with lid and 1.4 cm (0.6 in.) without lid
Length: 12.8 cm (5.0 in.)
Width: 8.6 cm (3.4 in.)

Well Bottom Area
0.25 cm²

Maximum Well Volume
350 µL

Recommended Maximum Working Volume
300 µL

Recommended Operating Vacuum
25.4 cm Hg (10 in. Hg) or greater

Filtration by Centrifugation
500 - 3,000 x g

Typical Sample Hold-up Volume
< 14 µL per well

Chemical Compatibility
See the Chemical Compatibility Chart.

Protein Recoveries For Different AcroPrep™ 96 MWCO Devices

MW (kDa) 10K 30K 100K

Vitamin B12 - 200 µg/mL 1.3 4 + 1% 5 + 1% 7 + 2%

Ovalbumin, turkey - 1.0 mg/mL 45 97 + 2% 98 + 0% 48 + 7%

IgG, goat - 1.0 mg/mL 160 98 + 1% 98 + 0% 96 + 1%

Apoferritin, equine - 1.0 mg/mL 443 96 + 1% 89 + 1% 93 + 0%

Observed Crosstalk in AcroPrep 96 Filter Plate = 0%

Fluorescein dye (200 µL of a 2 µg/mL H₂O stock) was added to wells of AcroPrep 96 plate with GHP membrane (5 plates total) in a checkerboard pattern. Alternate wells were filled with 200 µL of water. The fluid was evacuated from each filter plate using vacuum filtration at 30 cm Hg
(12 in. Hg) for 15 seconds and the filter plate read in a PerkinElmer, Wallac VICTOR* 1420 Multilabel Counter. Wells filled with water which show a CPS reading above the dashed line (5.2 x 10⁴ CPS, which is = “Average” Background + 5% Signal) in the graph constitute a crosstalk event.
CPS = Counts Per Second

AcroPrep 96 Filter Plates, 350 µL
Part Number	Description	Pkg	Price	Qty
5033	With Omega 3K membrane, natural, 350 µL well	10/pkg	318.15
5034	With Omega 10K membrane, natural, 350 µL well	10/pkg	318.15
5035	With Omega 30K membrane, natural, 350 µL well	10/pkg	318.15
5036	With Omega 100K membrane, natural, 350 µL well	10/pkg	318.15
5042	With 0.2 µm Bio-Inert membrane, natural, 350 µL well	10/pkg	152.71
5030	With 0.45 µm GHP membrane, natural, 350 µL well	10/pkg	197.16
5031	With 1.0 µm glass fiber, natural, 350 µL well	10/pkg	101.82
5032	With 1.0 µm glass fiber, white, 350 µL well	10/pkg	101.82
5039	With 1.2 µm Supor membrane, natural, 350 µL well	10/pkg	152.71
5041	With PP/1.2 µm Supor membrane, natural, 350 µL well	10/pkg	229.08
5046	With 3.0 µm glass fiber/0.2 µm Bio-Inert membrane, natural, 350 µL well	10/pkg	229.08
5047	With Mustang Q membrane, natural, 350 µL well	10/pkg	225.41
5048	With Mustang S membrane, natural, 350 µL well	10/pkg	225.41
5037	With 0.2 µm PTFE membrane, natural, 350 µL well	10/pkg	152.71
5038	With 0.45 µm PTFE membrane, natural, 350 µL well	10/pkg	152.71
5043	With 0.45 µm GHP membrane, white, 350 µL well, short tips	10/pkg	197.16
5044	With 0.45 µm GHP membrane, black, 350 µL well	10/pkg	197.16
5045	With 0.2 µm GHP membrane, natural, 350 µL well	10/pkg	197.16
7267	With 30-40 µm PP/PE mesh	10/pkg	292.69
Accessories
5017	Multi-well plate manifold	1/pkg	931.17
5028	Waste port adaptor kit for vacuum manifold	1/pkg	51.56
5225	Adapter collar for centrifugation	2/pkg	47.19

Contact Customer Service at 800-521-1520 for possible lead-time and date item will be available to ship.