Biodyne® Nylon Transfer Membranes

High sensitivity and low background for enhanced detection and resolution

Do not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing.
Intrinsically hydrophilic for easy wetting.
Superior performance with radioactive (Biodyne B membrane) and nonradioactive (Biodyne A membrane) detection systems.
Ideal for nucleic acid detection.

Four chemistries provide versatile adsorption properties:

Biodyne A Membrane (Amphoteric Nylon 6,6)
Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

Biodyne B Membrane (Positively-charged Nylon 6,6)
Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.

Biodyne C Membrane (Negatively-charged Nylon 6,6)
Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

Biodyne Plus Membrane (Positively-charged Nylon 6,6 with an extremely high isoelectric point)
With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

Media
Biodyne A Membrane: Amphoteric Nylon 6,6
Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
Biodyne C Membrane: Negatively-charged Nylon 6,6

Pore Size
0.2, 0.45, and 1.2 µm

Typical Thickness
152 µm (6.0 mils ± 0.5 mils)

Solvent Compatibility
Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatibile with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

In addition to standard sizes, these membranes are available in additional sizes. Pall Life Sciences can cut these membranes to your specifications. For information on sizes and cuts, call your local Pall Life Sciences office.

Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing

Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 1A - 4A: blot after (re)probing. 1B - 4B: blot after stripping, prior to re(probing).

Fluorescent Detection of DNA Using Biodyne Plus Membrane

ng total DNA

Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.

Biodyne A Membrane
Part Number	Description	Pkg	Price	Qty
60113	0.2 µm, 30 cm x 3 m roll	1/pkg	349.61
60102	0.45 µm, 82 mm discs	50/pkg	174.87
60103	0.45 µm, 85 mm discs	50/pkg	180.75
60104	0.45 µm, 132 mm discs	50/pkg	305.95
60105	0.45 µm, 137 mm discs	50/pkg	319.90
60101	0.45 µm, 7 x 8.5 cm sheets	10/pkg	91.31
60100	0.45 µm, 20 x 20 cm sheets	10/pkg	268.83
60120	0.45 µm, 20 cm x 3 m roll	1/pkg	260.92
60106	0.45 µm, 30 cm x 3 m roll	1/pkg	349.61
60108	1.2 µm, 30 cm x 3 m roll	1/pkg	349.61
Biodyne B Membrane, 0.45 µm
60202	82 mm discs	50/pkg	174.87
60203	85 mm discs	50/pkg	180.75
60204	132 mm discs	50/pkg	305.95
60205	137 mm discs	50/pkg	319.90
60201	7 x 8.5 cm sheets	10/pkg	91.31
60200	20 x 20 cm sheets	10/pkg	266.19
60209	20 cm x 1 m roll	1/pkg	135.72
60208	20 cm x 3 m roll	1/pkg	260.92
60207	30 cm x 3 m roll	1/pkg	349.61
Biodyne C Membrane, 0.45 µm
60316	82 mm discs	50/pkg	174.87
60317	85 mm discs	50/pkg	180.75
60318	132 mm discs	50/pkg	307.43
60319	137 mm discs	50/pkg	319.90
60315	7 x 8.5 cm sheets	10/pkg	91.31
60314	20 x 20 cm sheets	10/pkg	266.19
Biodyne Plus Membrane, 0.45 µm
60402	82 mm discs	50/pkg	174.87
60403	85 mm discs	50/pkg	180.75
60404	132 mm discs	50/pkg	278.10
60405	137 mm discs	50/pkg	319.90
60401	7 x 8.5 cm sheets	10/pkg	91.31
60400	20 x 20 cm sheets	10/pkg	266.19
60406	30 cm x 3 m roll	1/pkg	349.61

Contact Customer Service at 800-521-1520 for possible lead-time and date item will be available to ship.